Publications
1: Lubin JW, Rao T, Mandell EK, Wuttke DS, Lundblad V. Dissecting Protein
Function: An Efficient Protocol for Identifying Separation-of-Function Mutations
That Encode Structurally Stable Proteins. Genetics. 2013 Jan 10. [Epub ahead of
print] PubMed PMID: 23307900.
2: Dickey TH, McKercher MA, Wuttke DS. Nonspecific Recognition Is Achieved in
Pot1pC through the Use of Multiple Binding Modes. Structure. 2013 Jan
8;21(1):121-32. doi: 10.1016/j.str.2012.10.015. Epub 2012 Nov 29. PubMed PMID:
23201273; PubMed Central PMCID: PMC3545015.
3: Altschuler SE, Croy JE, Wuttke DS. A small molecule inhibitor of Pot1 binding
to telomeric DNA. Biochemistry. 2012 Oct 9;51(40):7833-45. doi:
10.1021/bi300365k. Epub 2012 Sep 26. PubMed PMID: 22978652; PubMed Central PMCID:
PMC3514547.
4: Lewis KA, Wuttke DS. Telomerase and telomere-associated proteins: structural
insights into mechanism and evolution. Structure. 2012 Jan 11;20(1):28-39. doi:
10.1016/j.str.2011.10.017. Review. PubMed PMID: 22244753.
5: Braun LJ, Eldridge AM, Cummiskey J, Arthur KK, Wuttke DS. The role of adjuvant
in mediating antigen structure and stability. J Pharm Sci. 2012
Apr;101(4):1391-9. doi: 10.1002/jps.23039. Epub 2011 Dec 28. PubMed PMID:
22213631.
6: Altschuler SE, Dickey TH, Wuttke DS. Schizosaccharomyces pombe protection of
telomeres 1 utilizes alternate binding modes to accommodate different telomeric
sequences. Biochemistry. 2011 Sep 6;50(35):7503-13. doi: 10.1021/bi200826a. Epub
2011 Aug 16. PubMed PMID: 21815629; PubMed Central PMCID: PMC3184176.
7: Mandell EK, Gelinas AD, Wuttke DS, Lundblad V. Sequence-specific binding to
telomeric DNA is not a conserved property of the Cdc13 DNA binding domain.
Biochemistry. 2011 Jul 26;50(29):6289-91. doi: 10.1021/bi2005448. Epub 2011 Jun
29. PubMed PMID: 21668015.
8: Alford JR, Fowler AC, Wuttke DS, Kerwin BA, Latypov RF, Carpenter JF, Randolph
TW. Effect of benzyl alcohol on recombinant human interleukin-1 receptor
antagonist structure and hydrogen-deuterium exchange. J Pharm Sci. 2011 May 5.
doi: 10.1002/jps.22601. [Epub ahead of print] PubMed PMID: 21557223.
9: Lee J, Mandell EK, Rao T, Wuttke DS, Lundblad V. Investigating the role of the
Est3 protein in yeast telomere replication. Nucleic Acids Res. 2010
Apr;38(7):2279-90. doi: 10.1093/nar/gkp1173. Epub 2010 Jan 4. PubMed PMID:
20047960; PubMed Central PMCID: PMC2853109.
10: Gelinas AD, Paschini M, Reyes FE, Héroux A, Batey RT, Lundblad V, Wuttke DS.
Telomere capping proteins are structurally related to RPA with an additional
telomere-specific domain. Proc Natl Acad Sci U S A. 2009 Nov
17;106(46):19298-303. doi: 10.1073/pnas.0909203106. Epub 2009 Nov 2. PubMed PMID:
19884503; PubMed Central PMCID: PMC2780795.
11: Croy JE, Altschuler SE, Grimm NE, Wuttke DS. Nonadditivity in the recognition
of single-stranded DNA by the schizosaccharomyces pombe protection of telomeres 1
DNA-binding domain, Pot1-DBD. Biochemistry. 2009 Jul 28;48(29):6864-75. doi:
10.1021/bi900307x. PubMed PMID: 19518131; PubMed Central PMCID: PMC2756145.
12: Croy JE, Wuttke DS. Insights into the dynamics of specific telomeric
single-stranded DNA recognition by Pot1pN. J Mol Biol. 2009 Apr 10;387(4):935-48.
doi: 10.1016/j.jmb.2009.02.016. Epub 2009 Feb 13. PubMed PMID: 19232358.
13: Zappulla DC, Roberts JN, Goodrich KJ, Cech TR, Wuttke DS. Inhibition of yeast
telomerase action by the telomeric ssDNA-binding protein, Cdc13p. Nucleic Acids
Res. 2009 Feb;37(2):354-67. doi: 10.1093/nar/gkn830. Epub 2008 Nov 29. PubMed
PMID: 19043074; PubMed Central PMCID: PMC2632905.
14: Croy JE, Fast JL, Grimm NE, Wuttke DS. Deciphering the mechanism of
thermodynamic accommodation of telomeric oligonucleotide sequences by the
Schizosaccharomyces pombe protection of telomeres 1 (Pot1pN) protein.
Biochemistry. 2008 Apr 15;47(15):4345-58. doi: 10.1021/bi701778x. Epub 2008 Mar
21. PubMed PMID: 18355038.
15: Theobald DL, Wuttke DS. Accurate structural correlations from maximum
likelihood superpositions. PLoS Comput Biol. 2008 Feb;4(2):e43. doi:
10.1371/journal.pcbi.0040043. PubMed PMID: 18282091; PubMed Central PMCID:
PMC2242818.
16: Eldridge AM, Wuttke DS. Probing the mechanism of recognition of ssDNA by the
Cdc13-DBD. Nucleic Acids Res. 2008 Mar;36(5):1624-33. doi: 10.1093/nar/gkn017.
Epub 2008 Feb 3. PubMed PMID: 18250086; PubMed Central PMCID: PMC2275150.
17: Alford JR, Kwok SC, Roberts JN, Wuttke DS, Kendrick BS, Carpenter JF,
Randolph TW. High concentration formulations of recombinant human interleukin-1
receptor antagonist: I. Physical characterization. J Pharm Sci. 2008
Aug;97(8):3035-50. PubMed PMID: 17973297.
18: Strauss DM, Wuttke DS. Characterization of protein-protein interactions
critical for poliovirus replication: analysis of 3AB and VPg binding to the
RNA-dependent RNA polymerase. J Virol. 2007 Jun;81(12):6369-78. Epub 2007 Apr 4.
PubMed PMID: 17409142; PubMed Central PMCID: PMC1900118.
19: Theobald DL, Wuttke DS. Empirical Bayes hierarchical models for regularizing
maximum likelihood estimation in the matrix Gaussian Procrustes problem. Proc
Natl Acad Sci U S A. 2006 Dec 5;103(49):18521-7. Epub 2006 Nov 27. PubMed PMID:
17130458; PubMed Central PMCID: PMC1664551.
20: Croy JE, Wuttke DS. Themes in ssDNA recognition by telomere-end protection
proteins. Trends Biochem Sci. 2006 Sep;31(9):516-25. Epub 2006 Aug 4. Review.
PubMed PMID: 16890443.
21: Croy JE, Podell ER, Wuttke DS. A new model for Schizosaccharomyces pombe
telomere recognition: the telomeric single-stranded DNA-binding activity of
Pot11-389. J Mol Biol. 2006 Aug 4;361(1):80-93. Epub 2006 Jun 19. Erratum in: J
Mol Biol. 2006 Oct 6;362(5):1181. PubMed PMID: 16842820.
22: Theobald DL, Wuttke DS. THESEUS: maximum likelihood superpositioning and
analysis of macromolecular structures. Bioinformatics. 2006 Sep 1;22(17):2171-2.
Epub 2006 Jun 15. PubMed PMID: 16777907; PubMed Central PMCID: PMC2584349.
23: Eldridge AM, Halsey WA, Wuttke DS. Identification of the determinants for the
specific recognition of single-strand telomeric DNA by Cdc13. Biochemistry. 2006
Jan 24;45(3):871-9. PubMed PMID: 16411763; PubMed Central PMCID: PMC3514546.
24: Theobald DL, Wuttke DS. Divergent evolution within protein superfolds
inferred from profile-based phylogenetics. J Mol Biol. 2005 Dec 2;354(3):722-37.
Epub 2005 Sep 20. PubMed PMID: 16266719; PubMed Central PMCID: PMC1769326.
25: Jacobs SA, Podell ER, Wuttke DS, Cech TR. Soluble domains of telomerase
reverse transcriptase identified by high-throughput screening. Protein Sci. 2005
Aug;14(8):2051-8. PubMed PMID: 16046627; PubMed Central PMCID: PMC2279316.
26: Theobald DL, Wuttke DS. Prediction of multiple tandem OB-fold domains in
telomere end-binding proteins Pot1 and Cdc13. Structure. 2004 Oct;12(10):1877-9.
PubMed PMID: 15458635.
27: Mitton-Fry RM, Anderson EM, Theobald DL, Glustrom LW, Wuttke DS. Structural
basis for telomeric single-stranded DNA recognition by yeast Cdc13. J Mol Biol.
2004 Apr 23;338(2):241-55. PubMed PMID: 15066429.
28: Wuttke DS. Targeting the end; the structure of the Ku80 C-terminal domain.
Structure. 2004 Mar;12(3):355-6. PubMed PMID: 15016349.
29: Theobald DL, Cervantes RB, Lundblad V, Wuttke DS. Homology among telomeric
end-protection proteins. Structure. 2003 Sep;11(9):1049-50. PubMed PMID:
12962623.
30: Strauss DM, Glustrom LW, Wuttke DS. Towards an understanding of the
poliovirus replication complex: the solution structure of the soluble domain of
the poliovirus 3A protein. J Mol Biol. 2003 Jul 4;330(2):225-34. PubMed PMID:
12823963.
31: Anderson EM, Halsey WA, Wuttke DS. Site-directed mutagenesis reveals the
thermodynamic requirements for single-stranded DNA recognition by the
telomere-binding protein Cdc13. Biochemistry. 2003 Apr 8;42(13):3751-8. PubMed
PMID: 12667066.
32: Theobald DL, Mitton-Fry RM, Wuttke DS. Nucleic acid recognition by OB-fold
proteins. Annu Rev Biophys Biomol Struct. 2003;32:115-33. Epub 2003 Feb 18.
Review. PubMed PMID: 12598368; PubMed Central PMCID: PMC1564333.
33: Ojennus DD, Lehto SE, Wuttke DS. Electrostatic interactions in the
reconstitution of an SH2 domain from constituent peptide fragments. Protein Sci.
2003 Jan;12(1):44-55. PubMed PMID: 12493827; PubMed Central PMCID: PMC2312404.
34: Anderson EM, Halsey WA, Wuttke DS. Delineation of the high-affinity
single-stranded telomeric DNA-binding domain of Saccharomyces cerevisiae Cdc13.
Nucleic Acids Res. 2002 Oct 1;30(19):4305-13. PubMed PMID: 12364610; PubMed
Central PMCID: PMC140553.
35: Glustrom LW, Mitton-Fry RM, Wuttke DS. Re:
1,1-Dichloro-2,2-bis-(p-chlorophenyl)ethylene and polychlorinated biphenyls and
breast cancer: combined analysis of five U.S. studies. J Natl Cancer Inst. 2002
Sep 4;94(17):1337-8; author reply 1338-9. PubMed PMID: 12208902.
36: Mitton-Fry RM, Wuttke DS. 1H, 13C and 15N resonance assignments of the
DNA-binding domain of the essential protein Cdc13 complexed with single-stranded
telomeric DNA. J Biomol NMR. 2002 Apr;22(4):379-80. PubMed PMID: 12018489.
37: Mitton-Fry RM, Anderson EM, Hughes TR, Lundblad V, Wuttke DS. Conserved
structure for single-stranded telomeric DNA recognition. Science. 2002 Apr
5;296(5565):145-7. PubMed PMID: 11935027.
38: Ojennus DD, Fleissner MR, Wuttke DS. Reconstitution of a native-like SH2
domain from disordered peptide fragments examined by multidimensional
heteronuclear NMR. Protein Sci. 2001 Nov;10(11):2162-75. PubMed PMID: 11604523;
PubMed Central PMCID: PMC2374061.
39: Dahlke Ojennus D, Mitton-Fry RM, Wuttke DS. Induced alignment and measurement
of dipolar couplings of an SH2 domain through direct binding with filamentous
phage. J Biomol NMR. 1999 Jun;14(2):175-9. doi: 10.1023/A:1008304332574. PubMed
PMID: 21080256.
Function: An Efficient Protocol for Identifying Separation-of-Function Mutations
That Encode Structurally Stable Proteins. Genetics. 2013 Jan 10. [Epub ahead of
print] PubMed PMID: 23307900.
2: Dickey TH, McKercher MA, Wuttke DS. Nonspecific Recognition Is Achieved in
Pot1pC through the Use of Multiple Binding Modes. Structure. 2013 Jan
8;21(1):121-32. doi: 10.1016/j.str.2012.10.015. Epub 2012 Nov 29. PubMed PMID:
23201273; PubMed Central PMCID: PMC3545015.
3: Altschuler SE, Croy JE, Wuttke DS. A small molecule inhibitor of Pot1 binding
to telomeric DNA. Biochemistry. 2012 Oct 9;51(40):7833-45. doi:
10.1021/bi300365k. Epub 2012 Sep 26. PubMed PMID: 22978652; PubMed Central PMCID:
PMC3514547.
4: Lewis KA, Wuttke DS. Telomerase and telomere-associated proteins: structural
insights into mechanism and evolution. Structure. 2012 Jan 11;20(1):28-39. doi:
10.1016/j.str.2011.10.017. Review. PubMed PMID: 22244753.
5: Braun LJ, Eldridge AM, Cummiskey J, Arthur KK, Wuttke DS. The role of adjuvant
in mediating antigen structure and stability. J Pharm Sci. 2012
Apr;101(4):1391-9. doi: 10.1002/jps.23039. Epub 2011 Dec 28. PubMed PMID:
22213631.
6: Altschuler SE, Dickey TH, Wuttke DS. Schizosaccharomyces pombe protection of
telomeres 1 utilizes alternate binding modes to accommodate different telomeric
sequences. Biochemistry. 2011 Sep 6;50(35):7503-13. doi: 10.1021/bi200826a. Epub
2011 Aug 16. PubMed PMID: 21815629; PubMed Central PMCID: PMC3184176.
7: Mandell EK, Gelinas AD, Wuttke DS, Lundblad V. Sequence-specific binding to
telomeric DNA is not a conserved property of the Cdc13 DNA binding domain.
Biochemistry. 2011 Jul 26;50(29):6289-91. doi: 10.1021/bi2005448. Epub 2011 Jun
29. PubMed PMID: 21668015.
8: Alford JR, Fowler AC, Wuttke DS, Kerwin BA, Latypov RF, Carpenter JF, Randolph
TW. Effect of benzyl alcohol on recombinant human interleukin-1 receptor
antagonist structure and hydrogen-deuterium exchange. J Pharm Sci. 2011 May 5.
doi: 10.1002/jps.22601. [Epub ahead of print] PubMed PMID: 21557223.
9: Lee J, Mandell EK, Rao T, Wuttke DS, Lundblad V. Investigating the role of the
Est3 protein in yeast telomere replication. Nucleic Acids Res. 2010
Apr;38(7):2279-90. doi: 10.1093/nar/gkp1173. Epub 2010 Jan 4. PubMed PMID:
20047960; PubMed Central PMCID: PMC2853109.
10: Gelinas AD, Paschini M, Reyes FE, Héroux A, Batey RT, Lundblad V, Wuttke DS.
Telomere capping proteins are structurally related to RPA with an additional
telomere-specific domain. Proc Natl Acad Sci U S A. 2009 Nov
17;106(46):19298-303. doi: 10.1073/pnas.0909203106. Epub 2009 Nov 2. PubMed PMID:
19884503; PubMed Central PMCID: PMC2780795.
11: Croy JE, Altschuler SE, Grimm NE, Wuttke DS. Nonadditivity in the recognition
of single-stranded DNA by the schizosaccharomyces pombe protection of telomeres 1
DNA-binding domain, Pot1-DBD. Biochemistry. 2009 Jul 28;48(29):6864-75. doi:
10.1021/bi900307x. PubMed PMID: 19518131; PubMed Central PMCID: PMC2756145.
12: Croy JE, Wuttke DS. Insights into the dynamics of specific telomeric
single-stranded DNA recognition by Pot1pN. J Mol Biol. 2009 Apr 10;387(4):935-48.
doi: 10.1016/j.jmb.2009.02.016. Epub 2009 Feb 13. PubMed PMID: 19232358.
13: Zappulla DC, Roberts JN, Goodrich KJ, Cech TR, Wuttke DS. Inhibition of yeast
telomerase action by the telomeric ssDNA-binding protein, Cdc13p. Nucleic Acids
Res. 2009 Feb;37(2):354-67. doi: 10.1093/nar/gkn830. Epub 2008 Nov 29. PubMed
PMID: 19043074; PubMed Central PMCID: PMC2632905.
14: Croy JE, Fast JL, Grimm NE, Wuttke DS. Deciphering the mechanism of
thermodynamic accommodation of telomeric oligonucleotide sequences by the
Schizosaccharomyces pombe protection of telomeres 1 (Pot1pN) protein.
Biochemistry. 2008 Apr 15;47(15):4345-58. doi: 10.1021/bi701778x. Epub 2008 Mar
21. PubMed PMID: 18355038.
15: Theobald DL, Wuttke DS. Accurate structural correlations from maximum
likelihood superpositions. PLoS Comput Biol. 2008 Feb;4(2):e43. doi:
10.1371/journal.pcbi.0040043. PubMed PMID: 18282091; PubMed Central PMCID:
PMC2242818.
16: Eldridge AM, Wuttke DS. Probing the mechanism of recognition of ssDNA by the
Cdc13-DBD. Nucleic Acids Res. 2008 Mar;36(5):1624-33. doi: 10.1093/nar/gkn017.
Epub 2008 Feb 3. PubMed PMID: 18250086; PubMed Central PMCID: PMC2275150.
17: Alford JR, Kwok SC, Roberts JN, Wuttke DS, Kendrick BS, Carpenter JF,
Randolph TW. High concentration formulations of recombinant human interleukin-1
receptor antagonist: I. Physical characterization. J Pharm Sci. 2008
Aug;97(8):3035-50. PubMed PMID: 17973297.
18: Strauss DM, Wuttke DS. Characterization of protein-protein interactions
critical for poliovirus replication: analysis of 3AB and VPg binding to the
RNA-dependent RNA polymerase. J Virol. 2007 Jun;81(12):6369-78. Epub 2007 Apr 4.
PubMed PMID: 17409142; PubMed Central PMCID: PMC1900118.
19: Theobald DL, Wuttke DS. Empirical Bayes hierarchical models for regularizing
maximum likelihood estimation in the matrix Gaussian Procrustes problem. Proc
Natl Acad Sci U S A. 2006 Dec 5;103(49):18521-7. Epub 2006 Nov 27. PubMed PMID:
17130458; PubMed Central PMCID: PMC1664551.
20: Croy JE, Wuttke DS. Themes in ssDNA recognition by telomere-end protection
proteins. Trends Biochem Sci. 2006 Sep;31(9):516-25. Epub 2006 Aug 4. Review.
PubMed PMID: 16890443.
21: Croy JE, Podell ER, Wuttke DS. A new model for Schizosaccharomyces pombe
telomere recognition: the telomeric single-stranded DNA-binding activity of
Pot11-389. J Mol Biol. 2006 Aug 4;361(1):80-93. Epub 2006 Jun 19. Erratum in: J
Mol Biol. 2006 Oct 6;362(5):1181. PubMed PMID: 16842820.
22: Theobald DL, Wuttke DS. THESEUS: maximum likelihood superpositioning and
analysis of macromolecular structures. Bioinformatics. 2006 Sep 1;22(17):2171-2.
Epub 2006 Jun 15. PubMed PMID: 16777907; PubMed Central PMCID: PMC2584349.
23: Eldridge AM, Halsey WA, Wuttke DS. Identification of the determinants for the
specific recognition of single-strand telomeric DNA by Cdc13. Biochemistry. 2006
Jan 24;45(3):871-9. PubMed PMID: 16411763; PubMed Central PMCID: PMC3514546.
24: Theobald DL, Wuttke DS. Divergent evolution within protein superfolds
inferred from profile-based phylogenetics. J Mol Biol. 2005 Dec 2;354(3):722-37.
Epub 2005 Sep 20. PubMed PMID: 16266719; PubMed Central PMCID: PMC1769326.
25: Jacobs SA, Podell ER, Wuttke DS, Cech TR. Soluble domains of telomerase
reverse transcriptase identified by high-throughput screening. Protein Sci. 2005
Aug;14(8):2051-8. PubMed PMID: 16046627; PubMed Central PMCID: PMC2279316.
26: Theobald DL, Wuttke DS. Prediction of multiple tandem OB-fold domains in
telomere end-binding proteins Pot1 and Cdc13. Structure. 2004 Oct;12(10):1877-9.
PubMed PMID: 15458635.
27: Mitton-Fry RM, Anderson EM, Theobald DL, Glustrom LW, Wuttke DS. Structural
basis for telomeric single-stranded DNA recognition by yeast Cdc13. J Mol Biol.
2004 Apr 23;338(2):241-55. PubMed PMID: 15066429.
28: Wuttke DS. Targeting the end; the structure of the Ku80 C-terminal domain.
Structure. 2004 Mar;12(3):355-6. PubMed PMID: 15016349.
29: Theobald DL, Cervantes RB, Lundblad V, Wuttke DS. Homology among telomeric
end-protection proteins. Structure. 2003 Sep;11(9):1049-50. PubMed PMID:
12962623.
30: Strauss DM, Glustrom LW, Wuttke DS. Towards an understanding of the
poliovirus replication complex: the solution structure of the soluble domain of
the poliovirus 3A protein. J Mol Biol. 2003 Jul 4;330(2):225-34. PubMed PMID:
12823963.
31: Anderson EM, Halsey WA, Wuttke DS. Site-directed mutagenesis reveals the
thermodynamic requirements for single-stranded DNA recognition by the
telomere-binding protein Cdc13. Biochemistry. 2003 Apr 8;42(13):3751-8. PubMed
PMID: 12667066.
32: Theobald DL, Mitton-Fry RM, Wuttke DS. Nucleic acid recognition by OB-fold
proteins. Annu Rev Biophys Biomol Struct. 2003;32:115-33. Epub 2003 Feb 18.
Review. PubMed PMID: 12598368; PubMed Central PMCID: PMC1564333.
33: Ojennus DD, Lehto SE, Wuttke DS. Electrostatic interactions in the
reconstitution of an SH2 domain from constituent peptide fragments. Protein Sci.
2003 Jan;12(1):44-55. PubMed PMID: 12493827; PubMed Central PMCID: PMC2312404.
34: Anderson EM, Halsey WA, Wuttke DS. Delineation of the high-affinity
single-stranded telomeric DNA-binding domain of Saccharomyces cerevisiae Cdc13.
Nucleic Acids Res. 2002 Oct 1;30(19):4305-13. PubMed PMID: 12364610; PubMed
Central PMCID: PMC140553.
35: Glustrom LW, Mitton-Fry RM, Wuttke DS. Re:
1,1-Dichloro-2,2-bis-(p-chlorophenyl)ethylene and polychlorinated biphenyls and
breast cancer: combined analysis of five U.S. studies. J Natl Cancer Inst. 2002
Sep 4;94(17):1337-8; author reply 1338-9. PubMed PMID: 12208902.
36: Mitton-Fry RM, Wuttke DS. 1H, 13C and 15N resonance assignments of the
DNA-binding domain of the essential protein Cdc13 complexed with single-stranded
telomeric DNA. J Biomol NMR. 2002 Apr;22(4):379-80. PubMed PMID: 12018489.
37: Mitton-Fry RM, Anderson EM, Hughes TR, Lundblad V, Wuttke DS. Conserved
structure for single-stranded telomeric DNA recognition. Science. 2002 Apr
5;296(5565):145-7. PubMed PMID: 11935027.
38: Ojennus DD, Fleissner MR, Wuttke DS. Reconstitution of a native-like SH2
domain from disordered peptide fragments examined by multidimensional
heteronuclear NMR. Protein Sci. 2001 Nov;10(11):2162-75. PubMed PMID: 11604523;
PubMed Central PMCID: PMC2374061.
39: Dahlke Ojennus D, Mitton-Fry RM, Wuttke DS. Induced alignment and measurement
of dipolar couplings of an SH2 domain through direct binding with filamentous
phage. J Biomol NMR. 1999 Jun;14(2):175-9. doi: 10.1023/A:1008304332574. PubMed
PMID: 21080256.