Salmonella Pathogenesis

Salmonella induced cellular trafficking. Salmonella (magenta) infected macrophage labeled with pipB2 GFP11 (green). Retrograde transport of PipB2 labeled vesicles is observed followed by rapid anterograde movement to cell periphery. Click here...

Visualizing host-Cell Response to Salonella Invasion. GFP-Tagged Fusion proteions show clustering upon mCherry labeled Salmonella bacteria. Overlay highlighted.



Salmonella Effector Dynamics. PipB2 split-GFP fusion proteion displays dynamoic behavior in the context of the entire effector subset. PipB2-GFP1 SL1344 Salmonella-infected HeLa cell transiently expressing GFP1-10. PipB2 GFPcomp localizes with the SCV (center) and labels highly dynamic tubules protruding from this compartment. Images were acquired at 16 hours after infection (MOI = 50). Images were acquired every 10 s and movie displayed at 10 FPS. Scale bar, 10 microns. Click here...


Deflecting Polystyrene Beads with a Line-Shaped Optical Trap. At high enough intensities, one can impart optical forces onto microscopic objects. Here, polystyrene beads are traveling linearly within laminar flow. Upon hitting a line-shaped 1x100 micron tightly focused 'optical trap' they become deflected laterally across the microfluidic channel. Click here...



Optical Trap Trigger, Cell Capture, and Release. After performing quantitative and high-throughput microscopy on a HeLa suspension cell, a 1064 nm optical trap is triggered, captures the cells, and moves it across flow lamina to a collection port for enrichment.




Imaging Calcium (II) influx in neurons with a genetically encoded calcium sensor. This is a video showing the membrane-targeted cameleon sensor, LynD3cpv, expressed in hippocampal neurons. Glutamate stimulates a large influx of calcium. Spontaneous increases in calcium are readily detectable before glutamate addition. The z-axis represents the ratio of the cameleon sensor, where a high ratio correlates with increases in calcium (II).